Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay
نویسندگان
چکیده
منابع مشابه
Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.
The effects of serum on the expression of four commonly used housekeeping genes were examined in serum-stimulated fibroblasts in order to validate the internal control genes for a quantitative RT-PCR assay. NIH 3T3 fibroblasts transfected with an inducible chimeric gene were serum-starved for 24 h and then induced with 15% serum for 8 h. Serum did not alter the amount of total RNA that was expr...
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Quantitative real-time PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistic...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2009
ISSN: 1471-2164
DOI: 10.1186/1471-2164-10-491